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. 2017 Aug 7;127(9):3392–3401. doi: 10.1172/JCI94027

Figure 4. Cyp27a1 deficiency does not affect hematopoiesis under steady-state conditions.

Figure 4

(A) Plasma 27HC levels in WT and Cyp27a1–/– mice (a total of 3 mice/treatment from 3 independent experiments). (B) Colony-forming progenitors in the blood of WT and Cyp27a1–/– mice (a total of 6 mice/genotype from 3 independent experiments). (C) Incorporation of a 7-day pulse of BrdU by HSCs or whole bone marrow cells from WT or Cyp27a1–/– male or female mice (a total of 3 mice/treatment from 3 independent experiments). (D and E) The numbers of hematopoietic stem and progenitor cells in the bone marrow (femurs and tibias; D) or spleen (E) of 3-month-old Cyp27a1–/– and littermate control mice (a total of 5 mice/genotype from 4 independent experiments). Statistical significance was assessed using 2-tailed unpaired Student’s t tests using the FDR method to correct for multiple comparisons, except in C, where we used 1-way ANOVA with Šídák’s multiple comparisons tests (*P < 0.05, ‡P < 0.01, #P < 0.001). All data represent mean ± SD.