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. 2017 Aug 7;127(9):3392–3401. doi: 10.1172/JCI94027

Figure 5. Pregnancy-induced HSC mobilization requires Cyp27a1, the enzyme that produces 27HC.

Figure 5

(A and B) The numbers of hematopoietic stem and progenitor cells in the bone marrow (femurs and tibias; A) and spleen (B) of WT non-pregnant mice, WT pregnant mice, and Cyp27a1–/– pregnant mice (a total of 4–5 mice/treatment from 5 independent experiments). Pregnant mice were analyzed on day 14.5 of gestation. (C) CFU in the blood of WT non-pregnant mice, WT pregnant mice, and Cyp27a1-/- pregnant mice (a total of 7 mice/treatment from 8 independent experiments). (D) Incorporation of a 24-hour pulse of BrdU by HSCs or unfractionated cells from the bone marrow or spleen of WT non-pregnant mice, WT pregnant mice, and Cyp27a1–/– pregnant mice (a total of 3–5 mice/treatment from 4 independent experiments). Statistical significance was assessed using 1-way ANOVA with Šídák’s multiple comparisons tests (*P < 0.05, ‡P < 0.01, #P < 0.001). All data represent mean ± SD.