Figure 4. T11 tumors express high levels of CXCL12.

(A) Whole-tumor RNA was isolated and analyzed using the PAMM-011 RT² Profiler PCR Array. Gene expression is presented relative to NT2 tumor controls. (B) Tumors were harvested on day 21 PTI, homogenized, and analyzed by CXCL12 ELISA (n = 3–4 per group). (C) Serum was harvested on day 21 PTI and analyzed for CXCL12 ELISA (n = 3 per group). (D) Representative overlay of CXCR4 expression by CD4⁺FoxP3⁺ Tregs (Treg CD4), CD4⁺FoxP3^– T cells (Non-Treg CD4), and CD8⁺ T cells. (E) MFI values for the expression of CXCR4 from TILs isolated from mice with 20 mm² T11 tumors (n = 6). Statistical significance was determined by Student’s t test (B and C) or Mann-Whitney U test (E). Data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.