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. 2017 Aug 21;127(9):3287–3299. doi: 10.1172/JCI92955

Figure 1. H9-NSC graft morphology and Ki67 immunolabeling.

Figure 1

(A–E) Graft size was stable over time in the C5 hemisection lesion site, and grafts were well integrated with the host. GFP and GFAP double-labeling (horizontal sections). (F) Grafts nonsignificantly expanded from 1 to 3 months after grafting (P = 0.6, by ANOVA) and were stable in size thereafter. Data represent the mean ± SEM. (G) The total number of grafted human cells (detected by hNu, a human-specific cell marker) was significantly reduced at 3 and 6 months, but recovered by 12 and 18 months. P < 0.05, by ANOVA and **P < 0.001 and *P < 0.05, by Fisher’s exact post-hoc test. Data represent the mean ± SEM. (H–J) Cell proliferation was significantly reduced after 3 months. hNu indicates the human-specific nucleus marker; Ki67 labels proliferating cells. P < 0.0001, by ANOVA and ***P < 0.001 and **P < 0.01, by Fisher’s exact post-hoc test comparing results at 1 and 3 months with results at 6, 12, and 18 months, respectively. Data represent the mean ± SEM. For F, G, and J: 1 month, n = 3; 3 months, n = 3; 6 months, n = 5; 12 months, n = 3; and 18 months, n = 4. Scale bars: 550 μm (A–E); 7 μm (H and I).