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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nat Commun. 2014;5:3337. doi: 10.1038/ncomms4337

Figure 7. Ectopic expression of Olig2 in Gsx1-expressing progenitors.

Figure 7

(a) Schematic diagram of in utero electroporation. Plasmid DNA was injected into the fourth ventricle and electroporated to the Gsx1-expression region at E12.5. The electroporated brains were collected at E13.5 or E14.5. (b) Immunostaining of E13.5 wild-type cerebellum electroporated with Olig2 at E12.5. Antibodies are indicated. (c–f) Sections of E14.5 wild-type mice electroporated with (c,d) control and (e,f) Olig2. Regions just above the Gsx1-expressing cells are shown. Arrowheads indicate co-immunostaining of GFP and the marker. (g,h) Quantification of the percentages of (g) Pax2-positive cells and (h) Lhx1/5-positive cells in electroporated cells (n =3, NS, not significant, **P<0.01 by t-test, mean±s.e.m.). (i) Summary of this study. Scale bars represent (b) 25 μm, (inset of b,c–f) 10 μm.