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. 2017 Sep 26;102(6):1381–1388. doi: 10.1189/jlb.1A1116-463RR

Figure 3. Effect of DCs from colon or small intestine (SI) on T cell homing receptors and relation to retinoic acid production by the DCs.

Figure 3.

(A and B) OT-I CD8+ TCR transgenic T cells that were specific for the OVA peptide SIINFEKL were stimulated with this peptide and CD11c+ APCs from small intestine or colon as in Fig. 1. Four days later, T cells were stained for α4β7-integrin and CCR9 chemokine receptor and analyzed by flow cytometry. Both sources of DCs up-regulated α4β7, but only small intestinal DCs up-regulated CCR9. Representative flow plot (gates based on isotype controls; A), and means and ranges for 2 experiments using colon or SI CD11c+ APCs pooled from 3 mice each (B). (C) RALDH levels in DCs from small intestine or colon were measured by intracellular staining using an ALDEFluor assay (Materials and Methods), and geometric mean fluorescent index (GMFI) is plotted for 4 independent matched pairs of tissues from 4 independent experiments (P = 0.007). (D) RALDH mRNA levels by RT-PCR showing SI vs. colon CD11c+ APC pairs from 3 independent experiments of 3 mice each (P = 0.02).