Figure 9. CRF₁ potentiates Ca²⁺ mobilization from intracellular stores in MCs.

RBL-2H3 cells stably transfected with lentiviral CRF₁-overexpressed plasmid or empty control plasmid cells were stimulated with the sarco/endoplasmic reticulum Ca²⁺-ATPase inhibitor thapsigargin in the presence or absence of Ca²⁺, as described in the Materials and Methods section. (A) Peak Ca²⁺ responses (ΔF480/F540 ratio) induced by thapsigargin in RBL-2H3 cells under Ca²⁺-replete (1 mM Ca²⁺) (A) or Ca²⁺-free (B) media. Data shown are the means ± se and are representative of 4 independent experiments performed in triplicate. Data were analyzed using a 2-tailed t test. *P < 0.05.