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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: J Immunotoxicol. 2016 Jul 15;13(6):784–792. doi: 10.1080/1547691X.2016.1194917

Figure 6. Effect of G-CSF on differential numbers of WBC in gp10025–33-vaccinated mice.

Figure 6

Naive Ly5.2 mice were inoculated with 2.5 ×105 B16 cells in right flank and IP injected 10 days later (timepoint when tumor was established) with PBS or CTX and adoptively transferred with 106 un-fractionated naïve spleen cells from Ly5.1 donor pmel-1 Tg mice. Mice were injected one day post-PBS or -CTX treatment with G-CSF from Days 2-5. Mice were left without further manipulation or SC vaccinated with gp10025–33 ± poly(I:C) 5 days post-PBS or CTX treatment. Mice were then bled on Day 5 post-vaccination (Day 7 post-CTX treatment) to assess total numbers of (A) WBC, (B) lymphocytes, (C) monocytes, and (D) granulocytes in the peripheral blood via flow cytometry. *p ≤ 0.05 vs. prospective controls.