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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Gastroenterology. 2017 Aug 18;153(5):1338–1350.e3. doi: 10.1053/j.gastro.2017.08.024

Figure 3. TNF decreases mouse DRA but not mouse PAT-1 mRNA expression in crypt derived mouse ileal enteroids.

Figure 3

Crypt derived mouse Ileal enteroids grown on matrigel were treated for 6 or 24 h with TNF (50 ng/ml). Total RNA was extracted and quantitative real-time RT-PCR was performed utilizing SYBR green fluorescent dye. Data represent the relative expression of DRA (A) or PAT1 (B) normalized to the respective GAPDH mRNA (internal control) levels. Results are expressed as fold-change in mRNA levels in treated cells compared with control. Values are means ± SEM from ≥3 different experiments performed in triplicate. **p < 0.001 vs. control