Figure 7. In vivo effects of TNF on DRA expression in C57BL/6 mice.

TNF (5 μg/mouse in 200 μl PBS) or vehicle alone was injected i.p. to C57BL/6 mice. After 3 or 6 h, mice were sacrificed, intestines were removed and mucosa scraped from ileal and colonic regions for RNA isolation. Mouse DRA mRNA was measured by real-time PCR using gene specific primers. Relative DRA mRNA expression in ileal (A) and colonic mucosa (B) of mice treated with vehicle or TNF. Values are means ± SEM from 5 mice. **p < 0.001, *p < 0.05 vs. control. TNF decreases DRA immunostaining in Ileum (C) and colon (D). Green: DRA; Red: Villin; Blue: Nuclei.