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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Differentiation. 2017 Aug 1;96:70–81. doi: 10.1016/j.diff.2017.07.004

Figure 3. Longer differentiation supports more mature phenotype in myotubes.

Figure 3

(A) Representative image of MHC+ myotubes. From 2 to 6 weeks, single nucleated nascent myotubes migrated and fused to form larger multinucleated MHC+ myotubes. Scale bar = 50 μm. (B) The number of MHC+ cells tended to be increased at 6 weeks, although the difference did not reach statistically significance. (C) RT-PCR analysis of MYH genes in differentiated cells. Specific primers were used to detect the gene expression of embryonic (MYH3), fetal/perinatal (MYH8), and adult form of MHC (MYH2). (D) Immunocytochemistry of MYH3, MYH8, MYH2, and MHC (with MF20 antibody clone) in differentiated myotubes. Scale bar = 50 μm.