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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2017 Sep 1;16(11):2473–2485. doi: 10.1158/1535-7163.MCT-16-0834

Figure 4. 7rh reduced Ddr1-mediated tumorigenicity and signaling.

Figure 4

(A) 7rh inhibited DDR1-mediated signaling in a concentration-dependent manner in murine PDA cell line Pan02. Lysates were probed for the indicated targets by Western blot analysis. (B) Schematic representation of the animal experiment. Mouse Pan02 cell line was orthotopically injected into C57BL/6 mice and 7rh therapy of 25 mg/kg 3×/week started at day 19 and ended at day 40. (C) 7rh treatment reduced tumor burden compared to vehicle and led to a greater presence of normal acinar tissue (D, E). (F–H) Immunohistochemical analysis of tissue from each group depicted inhibition of Ddr1 activation and downstream signaling (P-Peak1, P-Pyk2), as well as enhanced cellular apoptosis (I) and reduced proliferation (J). Error bars: (*, p < 0.05; **, p < 0.005; ***, p < 0.0005; ****, p < 0.00005), one-way ANOVA with Tukey’s MCT.