Figure 4. Neutralization of TNF-α-mediated cellular cytotoxicity by bsAb.

L929 cells were incubated with 1 ng/ml of TNF-α and 4 μg/ml of actinomycin D. Then, cells were treated with various concentrations of bsAb, anti-TNFα mAb, anti-IL-17A mAb and anti-IGF-I/II mAb m708.5 as a control antibody. After 24 h, cell viabilities were determined by MTT method. The error bars represent mean ± SEM. Data shown are representatives of three independent experiments.