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. 2017 Jun 27;8(47):81880–81891. doi: 10.18632/oncotarget.18737

Figure 2. 3T3-A-EXO promoted 3LL tumor cell invasion in vitro.

Figure 2

(A) 3LL cells were pretreated with 30 μg/ml 3T3-EXO or 3T3-A-EXO for 4 h. Then, 3LL cell growth was detected at 24, 48 and 72 h by CCK-8 assay (n = 3). (B) 3LL cells were treated with 30 μg/ml 3T3-EXO or 3T3-A-EXO for 4 h. Then, the cells were plated in the top chamber of a Transwell plate. Twenty-four hours later, the number of cells on the bottom of the Transwell filter was imaged and quantified. (C) Statistical analysis of result B (n = 5). (D) 3LL cells were treated with 30 μg/ml 3T3-EXO or 3T3-A-EXO for 4 h. Then, the cells were plated in the top chamber precoated with 50 μl of Matrigel. Forty-eight hours later, the number of cells on the bottom of the Transwell filter was imaged and quantified. (E) Statistical analysis of result (D) (n = 5). (A, C, E) Results are presented as the mean ± SEM of three independent experiments. (B, D) One representative image out of five is presented. P-values were generated by one-way ANOVA followed by Tukey-Kramer multiple comparisons test; ***p< 0.001. Control indicates 3LL cells treated with PBS.