Skip to main content
. 2017 Jun 9;8(47):81910–81925. doi: 10.18632/oncotarget.18434

Figure 4. Analysis of cell proliferation, miR-143 and HK2 expression in human esophageal squamous cell carcinoma (ESCC) tissue by in situ hybridization and immunohistochemistry.

Figure 4

Representative hematoxylin and eosin [H&E]-stained and PCNA-stained ESCC tissues (2 cases are shown). miR-143 ISH signal (blue, 4-nitro-blue tetrazolium and 5-brom-4-chloro-3′-indolylphosphate; counterstain, nuclear fast red) was absent in ESCC tumor area. HK2 expression (brown, 3,3′-diaminobenzidine tetrahydrochloride) was moderate to strong in near serial formalin-fixed, paraffin-embedded sections of ESCC tumor tissue (Case 1 & 2). Scale bars: 50 μm (x200 magnification), and 25 μm (x400 magnification, inset) (n = 12 cases).