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. 2017 May 30;8(47):82006–82026. doi: 10.18632/oncotarget.18299

Figure 11. Rescue of sel-12 loss-of-function mutant phenotype in C. elegans.

Figure 11

(A) Schematic representation of C. elegans SEL-12 protein (human PSEN1 homolog). The Sel-12 mutant isoform was generated with the G361A mutation (analogous to human G382A) introduced into the conservative amino acid motif of SEL-12. The sel-12(ar171)unc-1 strain contains a W225Stop mutation that results in truncated protein lacking domains that are critical for SEL-12 proteolytic function. (B) Egg-laying defective (Egl) phenotype in sel-12(ar171)unc-1 strain. (C) The Egl phenotype is successfully rescued by injection of sel-12 wt and sel-12 G361A constructs but not by sur-5 GFP plasmid alone. Percentage of animals laying <15 or >15 eggs is shown. Number of analyzed animals for each strain: sel-12 wt, n=61; sel-12 G361A, n=51; 2 control strains: sur-5 GFP-1, n=50; sur-5 GFP-2, n=50.