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. 2017 May 30;8(47):82006–82026. doi: 10.18632/oncotarget.18299

Figure 4. Study of PSEN1 mutants in APP ε-cleavage assay.

Figure 4

(A, B) Co-transfections of wild type or mutant PSEN1 and APP695 bearing the Swedish mutation (ΔNL) were performed in PSEN1-/-/PSEN2-/- MEF cells. Cell-free intramembrane cleavage was induced by incubation of the samples at 37°C for 2 hours. APP C99 and γ-secretase cleavage product AICD were detected by APP CTF antibody (Sigma). No cleavage was observed for the control samples incubated on ice (B). mAb 22C11 antibody, which detects the full-length APP (APP FL), was used as a transfection control in the assay (B). (C) Histogram shows the percent ratio of AICD to uncleaved APP C99. P-value significance codes: ‘*’ <0.05.