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. 2017 May 30;8(47):82006–82026. doi: 10.18632/oncotarget.18299

Figure 7. Ce-IMP-2 protein is efficiently cleaving hPSEN1 substrates.

Figure 7

(A) Co-transfections of wild type C. elegans IMPAS and human PSEN1 constructs performed in HEK293 cells. Ce-IMP-2 protein, which is an orthologue of human IMP1, but not Ce-IMP-1 or Ce-IMP-3, is capable of cleaving hPSEN1 holoprotein, detected with N-terminal hPSEN1 antibody. (B) Co-expression of hPSEN1 CTF and hIMP1 or Ce-IMP-2 protein in PSEN1-/-, PSEN2-/- double knockout fibroblasts. Efficient cleavage of hPSEN1 C-terminal fragment by Ce-IMP-2, with higher efficiency than by human IMP1 was detected by antibody for an N-terminal epitope of hPSEN1 CTF.