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. 2017 May 30;8(47):82064–82077. doi: 10.18632/oncotarget.18289

Figure 2. Dual luciferase assay analysis of miR-423-5p binding to GSTM1 3′UTR.

Figure 2

(A) Predicting miR-423-5p binding sites in GSTM1 sequence by miRBase. (B) Relative luciferase activity in HK-2 cells after co-transfection with miR-423-5p mimics and pGL3-GSTM1-WT or pGL3- GSTM1-MUT. (C) Relative luciferase activity in HK-2 cells after co-transfection with miR-423-5p inhibitors and pGL3-GSTM1-WT or pGL3- GSTM1-MUT. Note: *denotes P < 0.05 in comparison with the NC group; GSTM1, Glutathione-S-transferase (GST) M1; miR-423-5p, microRNA-423-5p; HK-2 cells, human renal tubular epithelial cells; NC, negative control.