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. 2017 May 30;8(47):82064–82077. doi: 10.18632/oncotarget.18289

Figure 5. Analysis of oxidative stress in hypoxia/reoxygenation induced HK-2 cells.

Figure 5

(A) FACS analysis of cellular ROS in (1) normal control, (2) H/R, (3) negative control, (4) miR-423-5p mimics, (5) miR-423-5p inhibitor, (6) GSTM1, (7) siGSTM1 and (8) miR-423-5p inhibitor plus siGSTM1 groups of H/R induced HK-2 cells by intracellular DCHF-DA staining. (B) Analysis of cellular SOD activity in the 8 experimental HK-2 cell groups. (C) Analysis of cellular MDA activity in the 8 experimental HK-2 cell groups. (D) Analysis of cellular GST activity in the 8 experimental H/R induced HK-2 cell groups. (E) Expressions of SOD mRNA and protein in each group. (F) Protein band of SOD in each group by western blotting. Note: *denotes P < 0.05 compared with the normal control group; #denotes P < 0.05 compared with the H/R group; & denotes P < 0.05 compared with the miR-423-5p inhibitors group; miR-423-5p, microRNA-423-5p; GSTM1, Glutathione-S-transferase (GST) M1; RPTEC, renal proximal tubular epithelial cell; NC, negative control; H/R, hypoxia/reoxygenation; ROS, reactive oxygen species; SOD, superoxide dismutase; MDA, malondialdehyde; GST, glutathione S-transferase.