(A) Estimation of miR-423-5p and GSTM1 mRNA levels in the kidneys of sham and I/R group rats on days 1, 3, 7, 14 and 21 by qRT-PCR. (B) Quantification of GSTM1 protein levels in the renal tissues of sham and I/R group rats on days 1, 3, 7, 14 and 21 by analyzing gray values of western blots. (C) Western blotting analysis of GSTM1 in the renal tissues of sham and I/R group rats on days 1, 3, 7, 14 and 21 by western blotting. Note: *denotes P < 0.05 compared with the sham group; qRT-PCR, quantitative real-time polymerase chain reaction; miR-423-5p, microRNA-423-5p; GSTM1, Glutathione-S-transferase (GST) M1; RPTEC, renal proximal tubular epithelial cell; NC, negative control; I/R, ischemia/reperfusion.