Table 3. Transfection sequences and qRT-PCR primers.
Type | Sequence (5′–3′) |
---|---|
miR-423-5p (nonsense sequence) | CAGUACUUUUGUGUAGUA |
miR-423-5p mimics | UGAGGGGCAGAGAGCGAGACUUU |
miR-423-5p inhibitors | AAAGUCUCGCUCUCUGCCCCUCA |
siGSTM1 | CUCAAGCUAUGAGGAAAAGAAGU |
miR-423-5p primers | F: 5′- ATGGTTCGTGGGTGAGGGGCAGAGAGCGAGAGCA |
GGGTCCGAGGTATTCG -3′ | |
R: 5′- GTGCAGGGTCCGAGGT -3′ | |
GSTM1 primers | F: 5′- TTCCCAATCTGCCCTACTTG -3′ |
R: 5′-TCTCCTCTTCTGTCTCCCCA -3′ | |
U6 primers | F: 5′- CTCGCTTCGGCAGCACA -3′ |
F: 5′- AACGCTTCACGAATTTGCGT -3′ | |
SOD | F: 5′- AATGTGTCCATTGAAGATCGTGTGA -3′ |
R: 5′-GCTTCCAGCATTTCCAGTCTTTGTA -3′ | |
GAPDH | F: 5′- GACAACTTTGGCATCGTGGA -3′ |
R: 5′-ATGCAGGGATGATGTTCTGG -3′ |
Note: qRT-PCR, quantitative real-time polymerase chain reaction; miR-423-5p, microRNA-423-5p; GSTM1, Glutathione-S-transferase (GST) M1; SOD, superoxide dismutase; GAPDH, glyceraldehyde phosphate dehydrogenase.