Skip to main content
. 2017 May 30;8(47):82064–82077. doi: 10.18632/oncotarget.18289

Table 3. Transfection sequences and qRT-PCR primers.

Type Sequence (5′–3′)
miR-423-5p (nonsense sequence) CAGUACUUUUGUGUAGUA
miR-423-5p mimics UGAGGGGCAGAGAGCGAGACUUU
miR-423-5p inhibitors AAAGUCUCGCUCUCUGCCCCUCA
siGSTM1 CUCAAGCUAUGAGGAAAAGAAGU
miR-423-5p primers F: 5′- ATGGTTCGTGGGTGAGGGGCAGAGAGCGAGAGCA
GGGTCCGAGGTATTCG -3′
R: 5′- GTGCAGGGTCCGAGGT -3′
GSTM1 primers F: 5′- TTCCCAATCTGCCCTACTTG -3′
R: 5′-TCTCCTCTTCTGTCTCCCCA -3′
U6 primers F: 5′- CTCGCTTCGGCAGCACA -3′
F: 5′- AACGCTTCACGAATTTGCGT -3′
SOD F: 5′- AATGTGTCCATTGAAGATCGTGTGA -3′
R: 5′-GCTTCCAGCATTTCCAGTCTTTGTA -3′
GAPDH F: 5′- GACAACTTTGGCATCGTGGA -3′
R: 5′-ATGCAGGGATGATGTTCTGG -3′

Note: qRT-PCR, quantitative real-time polymerase chain reaction; miR-423-5p, microRNA-423-5p; GSTM1, Glutathione-S-transferase (GST) M1; SOD, superoxide dismutase; GAPDH, glyceraldehyde phosphate dehydrogenase.