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. 2017 Jul 28;8(47):82156–82164. doi: 10.18632/oncotarget.18955

Figure 3. Missense mutation in the AT-hook 1 domain of MeCP2 increased level of H3K9me2 in SH-SY5Y cells.

Figure 3

SH-SY5Y cells were transfected by lipofectin 2000 reagent with plasmids: control DsRed-Monomer-N1, pDsRed-MECP2B, pDsRed-MECP2B-R202H. (A) Cells confluence pictures taken at x200 under fluorescence images microscope with white light. (B) Red signals represent transfected cells expressed DsRed protein or MeCP2B-Red fused proteins. (C) Western blot analysis: Level of H3K9me2 increased in cells transfected with pDsRed-MECP2B-R202H but not with vector control or wt MECP2B. The levels of H3K9ac were no difference among three groups. (D) H3K9me2 or H3K9ac /Histone 3 (H3) intensity ratio, *: P < 0.05, mutant group compared to vector or wt group. Data were from three independent experiments.