Figure 3. Knockdown of Atg7 with siRNA decreases kaempferol-induced death in HepG2 cells.

Data are expressed as mean ± SD of at least three independent experiments. (A) Cell viability was measured by MTT assay and cell death was measured by LDH activity analysis. (B) Apoptotic rate was determined using flow cytometry and quantified the apoptotic rate. (C) Protein expression levels of cleaved caspase3 and β-actin were determined using western blot and quantified by densitometry.