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Data are expressed as mean ± SD of at least three independent experiments. (A) The mRNA expression levels of Atg5, Atg7 and Beclin1 were determined by qRT-PCR. (B) The protein expression levels of Atg5, Atg7, Beclin1, LC3B, p62and β-actin were determined by western blot and quantified by densitometry.
