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. 2017 Jul 19;8(47):82303–82313. doi: 10.18632/oncotarget.19398

Figure 6. EGFR knockdown suppresses prostate cancer cell proliferation and motility.

Figure 6

(A) CCK-8 assay. The relative cell viability of the Si-EGFR-treated groups of PC-3 and Du-145 cells was lower than that of NC-treated groups (cell viability of 0 nM was regarded as 1.0). (B) Colony-formation assay (representative wells are presented). The colony-formation rate was lower for Si-EGFR (50 nM)-transfected groups compared to NC (50 nM)-transfected groups. (C) Transwell assay (representative micrographs are presented). Si-EGFR (50 nM) impaired the motility of PC-3 cells. (D) Western blot analysis. Si-EGFR (50 nM) inhibited AKT/GSK-3β signaling-related protein expression and p-STAT3 in PC-3 and Du-145 cells. Error bars represent the S.E. obtained from three independent experiments; *P<0.05. Scale bar = 100 μm.