Figure 1. Immuno-staining of monocytes from patients with different types of epithelial cancers.

Monocytes were stained with antibodies to CD163 (green) and EpCAM (red), with nuclie stained with DAPI (blue) and imaged under a confocal microscope. Merged image is shown on the left. Monocytes were enriched from the blood of patients with different types of epithelial tumors using Ficoll Paque-plus density gradient centrifugation. Monocytes were from a patient with breast (A), cervical (B), ovarian (C), endometrial (D), and pancreatic (E) cancer as well as a healthy individual (F) negative control. Scale bar, 75μm.