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. 2017 Jul 18;8(47):82326–82343. doi: 10.18632/oncotarget.19320

Figure 2. Increased migratory and proliferative abilities in macrophages that have ingested cancer cells.

Figure 2

A Transwell migration assay was used to assay the migratory ability of macrophages that had or had not phagocytized apoptotic MCF-7 cells. (A) Representative microphotographs of migrating cells seen in control (no phagocytosis) or coculture (phagocytosis) cells. (B) Quantitative analysis of the numbers of cells on the lower surface of the membrane in the transwell plates. Membranes were fixed and dyed using 0.1% Crystal Violet and number of cells observed in each field was counted. Bars represent the average numbers of cell observed in 5 fields, ** p<0.01. (C) Changes in the numbers of macrophages that had (Coculture) or had not (CON) phagocytized apoptotic MCF-7 cells over 36 hours measured by the MTS assay. (D) Quantification of the percentages of cells in different phases of the cell cycle determined by flow cytometry. (E) Quantification of the percentage of macrophages in S/G2/M phases of the cell cycle by flow cytometry. Results shown are typical of the three independent experiments. (F) Change in the numbers of macrophages that had (Coculture) or had not (CON) phagocytized apoptotic MCF-7 cells over 6 days measured by the MTS assay. Data represent means ± S.E. (n=3). * p<0.05, ** p<0.01, *** p<0.001.