Figure 5. Secretion levels of candidate exosomal miRNA identified in the in vitro work in saliva from head and neck squamous cell carcinoma (HNSCC) patients and healthy controls.
The miRNA-sequencing data used to guide selection of ddPCR assay development based on reads per kilobase per million transcripts (RPKM) for exosomal (A) miR-486-5p, miR-486-5p_1, miR-486-3p, miR-486-3p_1, miR-122-5p, miR-143-3p, miR-451a, and miR-3591-3p, and (B) miR-10b-5p transcripts isolated from saliva samples obtained from a miRNA-sequencing data from a pilot study involving 5 HNSCC cases and 5 cancer-free controls. ddPCR results from TaqMan assays for (C) miR-10b-5p and (D) miR-486-5p performed on salivary exosomes isolates from HNSCC cases (n = 11) and controls (n = 9).