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. 2017 Jul 31;8(47):82491–82505. doi: 10.18632/oncotarget.19700

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Copyright: © 2017 Phan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A-D) HBEC cells treated with 3 μM of pioglitazone (P) or troglitazone (T) for 24 hours and followed by QPCR assay for mRNA expression of metabolic genes involved in lipid synthesis (A), lipid uptake (B), triglyceride metabolism (C) and β-oxidation (D). (E) Gene expression involved in lipid metabolism in various lung cell lines. Included are five lung cancer cell lines involving A549, H3255 Calu6, H1993, and H2347. During culture in the media supplemented with 5% NS or 5% CS, all cell lines were treated with pioglitazone 30 μM for 24 hours and followed by QPCR assay to profile mRNA expression of metabolic genes involved in lipid metabolism. Values are mean ± S.E.M. Statistical was analysis was performed using one-way ANOVA (Tukey’s post-tests). * p < 0.05; ^# p < 0.01; ^Δ p < 0.001.

(A-D) HBEC cells treated with 3 μM of pioglitazone (P) or troglitazone (T) for 24 hours and followed by QPCR assay for mRNA expression of metabolic genes involved in lipid synthesis (A), lipid uptake (B), triglyceride metabolism (C) and β-oxidation (D). (E) Gene expression involved in lipid metabolism in various lung cell lines. Included are five lung cancer cell lines involving A549, H3255 Calu6, H1993, and H2347. During culture in the media supplemented with 5% NS or 5% CS, all cell lines were treated with pioglitazone 30 μM for 24 hours and followed by QPCR assay to profile mRNA expression of metabolic genes involved in lipid metabolism. Values are mean ± S.E.M. Statistical was analysis was performed using one-way ANOVA (Tukey’s post-tests). * p < 0.05; ^# p < 0.01; ^Δ p < 0.001.