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. 2017 Aug 2;8(47):82754–82772. doi: 10.18632/oncotarget.19787

Figure 5. Effects of PATZ1 knockdown on the expression of proteinases, cell proliferation, migration, and invasion in DTC cell lines.

Figure 5

(A) The expression of PATZ1, uPA, and MMPs in DTC cell lines (TPC-1 and FTC-133) transfected with control siRNA (si-control) or siRNA targeting PATZ1 (si-PATZ1). A representative western blot is shown. SP1 and β-actin were used as internal loading control for nuclear extract and whole cell lysate, respectively. (B) Cell proliferation assay for TPC-1 cells transfected with si-control or si-PATZ1. The number of cells was counted every 24 h until 96 h after seeding the cells. (C) Cell proliferation assay for FTC-133 cells transfected with si-control or si-PATZ1. **p < 0.001. (D) Chamber migration assay for DTC cell lines transfected with si-control or si-PATZ1. The number of migrated cells is shown in the bar chart. (E) Chamber-invasion assay for DTC cell lines transfected with si-control or si-PATZ1. The cells that invaded through the transwell chamber coated with collagen type IV were counted as described in the Materials and Methods. The absorbance values are shown in the bar chart.