Figure 1. The effect of VEGF in lactate metabolism in AML cell lines.

¹H-¹³C-HSQC NMR spectra of HL60 (A), THP1 (B) and HEL (C) intracellular extracts cultured in RPMI (blue); RPMI with ¹³C-U-lactate (red), RPMI with 10μg/mL of VEGF (green) and RPMI with ¹³C-U-lactate and 10μg/mL of VEGF (black). (D) ¹H-NMR spectra highlight of the lactate methyl group when the three cell lines (HL60, THP1 and HEL) were cultured with ¹³C-U-lactate: in the absence and in the presence of VEGF. The percentage of intracellular ¹³C-lactate and ¹²C-lactate in each condition is indicated in the board. Gluc- glucose; Ace- acetate; Lac- lactate; Mal- malate; Ala- alanine; Glut- glutamate; Ade- adenosine; Suc- succinate; Ino- inositol; GSH- glutamyl moiety of glutathione and NT- ribosyl moiety of nucleotides. Results were obtained from 3 independent replicates, and representative figures are presented.