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. 2017 Aug 16;8(47):82803–82823. doi: 10.18632/oncotarget.20294

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Copyright: © 2017 Lopes-Coelho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Immunofluorescense and western blotting was performed in order to evaluate the effect of lactate and VEGF in the expression of MCT1 and MCT4, in HL60, THP1 and HEL cell lines. Immunofluorescense for the detection of MCT1 (A) and MCT4 (B), western bloting for MCT1 and MCT4 (C) which were respectively quantified (D and E) using control conditions of each cell line after normalization for β-actin and (F) evaluation of LDH isoenzymes in an agarose gel electrophoresis (LDH Isoenzymes Electrophoresis Kit; SRE612K, Interlab) and bands quantification in an EasyFix Interlab G26 equipment. C-Control, L-Lactate, V-VEGF, LV-NaLac plus VEGF. Error bars represent standard deviation; statistical significance **p<0.01, ***p<0.001. Results were obtained from 3 independent replicates, and representative figures are presented.

Immunofluorescense and western blotting was performed in order to evaluate the effect of lactate and VEGF in the expression of MCT1 and MCT4, in HL60, THP1 and HEL cell lines. Immunofluorescense for the detection of MCT1 (A) and MCT4 (B), western bloting for MCT1 and MCT4 (C) which were respectively quantified (D and E) using control conditions of each cell line after normalization for β-actin and (F) evaluation of LDH isoenzymes in an agarose gel electrophoresis (LDH Isoenzymes Electrophoresis Kit; SRE612K, Interlab) and bands quantification in an EasyFix Interlab G26 equipment. C-Control, L-Lactate, V-VEGF, LV-NaLac plus VEGF. Error bars represent standard deviation; statistical significance **p<0.01, ***p<0.001. Results were obtained from 3 independent replicates, and representative figures are presented.