Figure 3. miR-93-3p suppresses ccRCC cell apoptosis and promotes migration.

ACHN and 786-O cells were stained with Annexin V and PI, and apoptosis was analyzed via flow cytometry (A). 10⁴ cells were detected per sample. Apoptosis was detected by TUNEL staining after transfection for 48 h (B). Apoptotic cells are green, and nuclei are stained blue with DAPI. Wound healing assay showing transfected cell migration (C). Wound widths were normalized to NC-transfected cells. Wound healing percentage was calculated as: (wound width at 0 h - wound width at 48 h) / wound width at 0 h. ACHN and 786-O cell transwell migration (D) and invasion assays (E). ^*P<0.05, ^**P<0.001.