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. 2017 Aug 24;8(47):82824–82834. doi: 10.18632/oncotarget.20458

Figure 4. miR-93-3p directly targets PEDF.

Figure 4

PEDF expression was measured using qRT-PCR (A) and western blotting (B) after NC, miR-93-3p, or anti-miR-93-3p transfection for 48 h in ACHN and 786-O cells. Actin was used as a loading control in western blots. Experiments were repeated at least three times with duplicate samples. PEDF levels were measured in ccRCC patient samples via western blotting (C). Photomicrographs showing representative hematoxylin and eosin staining and PEDF immunohistochemical analysis results in human ccRCC and normal kidney tissues (D). The potential interaction between miR-93-3p and putative binding sites in the PEDF 3’-UTR. NC, miR-93-3p, or anti-miR-93-3p were co-transfected with the wild type PEDF 3’-UTR, Mut1, or Mut2 into HEK293 cells for 48 h. Luciferase activities were analyzed relative to the NC-transfected group (E). Data were presented as means ± SEM. Experiments (C-E) were repeated six times each with duplicate samples. *P<0.05, **P<0.001.