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. 2017 Aug 24;8(47):82824–82834. doi: 10.18632/oncotarget.20458

Figure 5. The role of PEDF in ccRCC cells.

Figure 5

ACHN and 786-O cells were transfected with control or PEDF vector and stained with Annexin V and PI, and apoptosis was analyzed via flow cytometry (A), transwell migration (B) and invasion assays (C) were performed in PEDF-transfected cells. Cell apoptosis was analyzed via flow cytometry after PEDF siRNA was co-transfected with anti-miR-93-3p into ACHN and 786-O cells (D). Co-transfected ACHN and 786-O cell transwell migration (E) and invasion assays (F). Apoptosis-related proteins (G) and MMP2/9 (H) were analyzed. All experiments were repeated at least three times with duplicate samples.*P<0.05, **P<0.001.