FIGURE 3.

Variation of the k_obs for cytochrome c reduction in function of the ionic strength for the WT and mutants of the soluble forms of human CPR. Cytochrome c reduction assays were performed in triplicate in 96 well microtiter plates as described in the Material and Methods section. (A) Salt dependent profile of the k_obs with the WT and the mutants. Symbols depicting the different mutants are included in the panel. (B) Maximal k_obs values were extracted from panel A and plotted as a function of the salt concentration. Symbols depicting the different mutants are identical as the ones in (A). k_obs values represent the average of three replicates and the error bars depict the standard deviation.