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. 2017 Nov 3;8:1313. doi: 10.1038/s41467-017-01483-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The structure of the full-length ZntB. a Side view, four subunits of ZntB pentamer are coloured grey, and one is coloured rainbow from blue (N-terminus) to red (C-terminus); the position of the membrane is indicated, trans membrane helices 1 and 2 as well as αβα-motif are labelled. b Top view (from periplasm) onto ZntB—10 trans membrane helices are arranged cylindrically, with TM2 ring at the periphery. Experimental density is contoured in blue. The connecting loops provide residues for the selectivity filter (in magenta), further exemplified in (c) structural comparison of selectivity filters from EcZntB (magenta) and TmCorA (cyan), only three out five monomers are shown (d). The overlay of Asn rings (of GxN motif) from ZntB (magenta), TmCorA (cyan) and MjCorA (yellow). Note that ZntB forms a slightly wider entry point to the pore