. 2017 Nov 3;7:14471. doi: 10.1038/s41598-017-14047-y

Table 2.

Validation of mRNA-Seq data of a selected set of wheat and Heterodera avenae genes with quantitative real-time PCR (qPCR) to confirm gene expression changes at the contact stage (treatment versus control).

Gene ID	Annotation	Log₂FC
Gene ID	Annotation	qPCR	mRNA-seq
Wheat DEGs
gene:TRAES3BF074000020CFD_g	Unnamed protein product [Triticum aestivum]	0.01	Inf (up)*
gene:Traes_1AS_F9013A945^a	Phenylalanine ammonia-lyase [Aegilops tauschii]	−0.76*	−1.17*
gene:Traes_2AL_8394449B2	Ubiquinol oxidase 1, mitochondrial (Precursor) [Nicotiana tabacum]	−1.19*	−1.27*
gene:Traes_2AS_EE549925C	Root peroxidase [T. aestivum]	−0.36	−0.67*
gene:Traes_3DL_EE0699FDC	Secologanin synthase [Ae. tauschii]	2.50*	2.80*
gene:Traes_6DS_768787FF4^a	Auxin-induced protein [Ae. tauschii]	−0.83*	−1.31*
gene:Traes_5bs_bcc1b9791^a	Respiratory burst oxidase homolog protein B [Oryza sativa subsp. japonica]	0.70*	0.73*
gene:Traes_4bl_eb96605ed^a	Agmatine coumaroyltransferase-2 [Hordeum vulgare]	−0.89*	−0.76*
gene:Traes_4al_dd83f1a44^a	Xylanase inhibitor [T. aestivum]	−0.92*	−0.71*
gene:Traes_2al_1a870ce7b^a	Probable aldo-keto reductase 3 [O. sativa subsp. japonica]	−0.56*	−0.61*
gene:Traes_4bs_63dd9d036^a	Lipoxygenase [T. aestivum]	0.21	−0.67*
gene:Traes_1bl_04b591073^a	NEP1-interacting protein 2 [Arabidopsis thaliana]	0.27	1.07*
Nematode DEGs
c73395.graph_c0	Macrophage migration inhibitory protein [Eriocheir sinensis]	−0.91	−2.00*
c62312.graph_c0	Programmed cell death protein 2 [Toxocara canis]	−2.32*	−1.05*
c73973.graph_c0	Neprilysin-2 [T. canis]	1.15*	1.08*
c78521.graph_c0	Fatty acyl-CoA desaturase, putative [Eimeria tenella]	2.42*	1.16*
c54125.graph_c0	Sialin [Ascaris suum]	1.99*	1.75*
c72543.graph_c0^b	Pectate lyase [Heterodera glycines]	2.17*	1.94*
c76930.graph_c0	Putative salivary protein [Culicoides sonorensis]	6.49*	2.09*
c79218.graph_c0	Predicted: transmembrane BAX inhibitor motif-containing protein 4-like [Amphimedon queenslandica]	Inf (up)	2.46*
c68622.graph_c0^b	Chitinase [H. glycines]	3.60*	3.32*
c78853.graph_c0^b	Cathepsin L2 [Sinonovacula constricta]	0.77*	1.83*

Inf (up) indicates that the expression of the gene was detected only in the treatment sample, but not the control sample. *P < 0.05. FC, fold change (treatment vs. control). ^aThe DEGs were involved in biotic stress pathways of wheat created using MapMan visualization. ^bThe DEGs were predicted effector genes in Heterodera avenae exposed to wheat roots.