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. 2017 Oct 30;8:775. doi: 10.3389/fphar.2017.00775

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Copyright © 2017 Chen, Xu, Zhang, Li, Cui, Li, Chang, Tang, Xu, Tian, Huang, Xu, Jin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TXL dose-dependently reduced the H/R induced death of HCMs. (A) CCK-8 assay evaluated the toxicity of TXL on HCMs under normal condition (n = 6 in each group, the cells incubated with medium without TXL were considered 100% viable). (B) CCK-8 assay evaluated the effects of TXL at different concentrations on HCMs during H/R (n = 4 in each group, the cells without treatment were considered 100% viable). (C) JC-1 assay evaluated the effects of TXL at different concentrations on the mitochondrial membrane potential of HCMs during H/R (n = 3 in each group). (D) Western blot analysis of cytochrome C (n = 3 in each group) ^*P < 0.05 vs. Normal; ^#P < 0.05 vs. 800 μg/mL TXL; ^$P < 0.05 vs. H/R; ^§P < 0.05 vs. H/R+100 μg/mL TXL; ✩P < 0.05 vs. H/R+200 μg/mL TXL.