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. 2017 Oct 30;8:774. doi: 10.3389/fphar.2017.00774

Figure 2.

Figure 2

Wnts, β-catenin, DKK1, LEF1, E-cadherin, α-SMA, COL-I, and FN expression in the MCsisolated from effluent of PD patients. (A) The identification of MCs isolated from effluent of PD patients with flow cytometry (cytokeratin+CD45−CD68−). (B) The shape transformation of HPMC. (C,C1–C7) The relative mRNA level of Wnt1, Wnt2, Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt7b,Wnt8a, Wnt10a, β-catenin, DKK1, LEF1, E-cadherin, α-SMA, COL-I, and FN in PD start group and PD > 1 year group. (D) The expression of β-catenin, E-cadherin, α-SMA, COL-I and β-actin in PD start group and PD>1 year group analyzed by western blotting. (E,E1–E4) The density of relative bands depicted of β-catenin, E-cadherin, α-SMA, COL-I by western blotting. Values are the mean ± s.e.m, n = 3, **P < 0.01, *P < 0.05 vs. control (PD-start group).