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. 2017 Oct 30;8:774. doi: 10.3389/fphar.2017.00774

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Copyright © 2017 Guo, Sun, Xiao, Gou, Fang, Liang, Wang, Li, Liu and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of Wnt related genes expression in cultured HPMCs under different glucose condition. (A) The shape transformation of HPMCs. (B,B1–B6) The relative mRNA level of Wnt1, Wnt5a, β-catenin, LEF1, E-cadherin, α-SMA, COL-I, and FN analyzed by real-time PCR. (C) The protein level of β-catenin, E-cadherin, α-SMA, COL-I analyzed by western blotting. (D,D1–D4) The density of relative bands depicted of β-catenin, E-cadherin, α-SMA, COL-I by western blotting. Values are the mean ± s.e.m., n = 3, ^**P < 0.01, ^*P < 0.05 vs. control (5.6 mM glucose).