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. 2017 Oct 30;8:774. doi: 10.3389/fphar.2017.00774

Figure 4.

Figure 4

Overexpression of DKK1 Blocked MMT Transformation in HPMCs Treated with High-Glucose. (A) Immunofluorescence microscopy of E-cadherin (green), α-SMA (green), and β-catenin (green) in HPMCs in negative control (NG), HG (High-Glucose), HG + pFlag-DDK1 and HG + pcDNA3.0 group. Nucleus is stained with DAPI: blue). Magnification objective, ×40. Scale bar: 25μm. (B,B1–B5) The relative mRNA level of β-catenin, LEF1, E-cadherin, α-SMA, COL-I, and FN analyzed by real-time PCR. (C) The protein level of β-catenin, E-cadherin, α-SMA, COL-I analyzed by western blotting. (D,D1–D4) The density of relative bands depicted of β-catenin, E-cadherin, α-SMA, COL-I by western blotting. Values are the mean ± s.e.m., n = 3, *P < 0.01 vs. control (NG), ##P < 0.01, #P < 0.05 vs. HG.