Figure 2.

Reprogramming of non-integrative iPSCs from UCs. (A) The workflow of UC-derived iPSC (UiPSC) reprogramming. The figure illustrates the reprogramming procedures (upper) and the shape change from UCs to iPSCs from day(D)0 to passage(P)10 (lower). Scale bar: 200 μm. (B) Example of successful establishment of an iPSC line. Scale bar: 200 μm. (C) Non-integration of UC-derived iPSCs. The left plasmid cartoons illustrate the two plasmid backbones and the inserted genes. The right image shows a PCR test for non-integration of these plasmids in established iPSC lines. By using UCs and H₂O as negative controls, and UCs at 3 days post-transfection as a positive control, PCR analysis showed that UiPSC lines had no integration of episomal plasmids.