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. 2017 Oct 30;10:348. doi: 10.3389/fnmol.2017.00348

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Copyright © 2017 Cheng, Lei, Yin, Wang, Jin and Xiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reprogramming of iNSCs from UCs and comparison of a successful iPSC reprogramming and an unsuccessful iPSC reprogramming. (A) The morphology of iNSCs derived from UCs. The upper panel shows the UC-derived iPSCs and iNSCs (UiNSCs) reprogrammed at the same time and the UiNSCs after single-colony replating. Scale bar: 100 μm. The middle panel shows higher-magnification images of iPSCs and iNSCs and the neural rosette of iNSCs. Scale bar: 200 μm. The lower panel shows the morphology comparison between iNSCs and iPSCs. Scale bar: 100 μm. (B) Immunofluorescent staining of iNSC markers SOX2, NESTIN, OLIG2, and PAX6, with nuclear DAPI counter-staining. Scale bar: 100 μm. (C) Compared with a successful iPSC reprogramming in the right panel, the left image shows that the UCs underwent shape transformation but were not fully reprogrammed. Some cells had semi-shape transformation (not round) and the clone cells were loosely attached to each other. Scale bar: 200 μm.