Table 1.

Stock and working concentrations of 4i compounds for regrogramming.

Compounds	CAS#	Molecular formula	Molecular weight	Stock fold	Stock concentration (mM)	Working concentration (μM)
A83-01	909910-43-6	C25H19N5S	421.52	10,000 ×	5	0.5
PD0325901	391210-10-9	C16H14F3IN2O4	482.19	10,000 ×	5	0.5
Thiazovivin	2656-71-8	C15H13N5OS	311.36162	10,000 ×	5	0.5
CHIR99021	252917-06-9	C22H18CI2N8	465.34	1,000 ×	3	3

Higher concentrations of chemical factors in this protocol may result in toxicity to the reprogrammed cells. CHIR99021 and A83-01 are fatal if swallowed, and can cause skin irritation, serious eye irritation and respiratory irritation. Avoid breathing these two compounds and contact with skin and eyes. PD0325901 is toxic if swallowed, and may cause damage to organs through prolonged or repeated exposure and long lasting harmful effects to aquatic life. Avoid releasing PD0325901 to the environment. Thiazovivin is not a hazardous substance or mixture. When dealing with the 4i compounds, do it in a well-ventilated area. Wear protective gloves, eye protection, and face protection.