Figure 2.
Comparison of the production kinetics between IFN-γ and ET-1 in the culture supernatant of PBMCs stimulated with immobilized anti-CD3 Ab. ET1 production in the culture supernatant of 1 × 106 cells/well of human PBMCs from a healthy donor cultured with 10 μg/ml immobilized anti-human CD3 Ab. The culture supernatants at appropriate times were collected and measured by ET-1 or IFN-γ ELISA. Assays were performed in triplicate wells, and data represent means ± SEM. ND = not detected. * P < 0.001 as calculated compared with negative control (Time 0) by two-way ANOVA followed by post-hoc Sediak’s multiple comparison test. Similar results were obtained in 3 different experiments.