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. 2017 Nov 3;7:14500. doi: 10.1038/s41598-017-14202-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of antigen-specific activation of T cells on monocytes in mice. Three million Tax11-19-specific CTLs were stimulated with 4 × 10⁶ mitomycin C-treated syngeneic spleen cells from HHD mice in the presence or absence of 0.1 μM Tax11-19 peptide as an antigen (Ag) in the outer chamber. In the inner chamber, after bone marrow cells were prepared from 4 mice of femurs in each strain, 1.5 × 10⁶ /well of monocytes purified with Mini-MACS from bone marrow cells of (a) HHD mice and (b) ET1^flox/flox;Tie2-Cre(+), ET1^flox/+;Tie2-Cre(+), and ET1^flox/flox;Tie2-Cre(−) mice were cultured for 24 h. The concentration of each blocking Ab used was 30 µg/ml. ND = not detected. The assays were performed in triplicate wells. Data are expressed as the mean ± SEM. ***p < 0.001 as compared with positive (a) or negative (b) control by one-way ANOVA followed by post-hoc Tukey’s multiple comparison test. Similar results were obtained in two experiments (a,b).