Table 1.
Characteristics of FGF2 and IGF1 molecular masses detected by mass spectrometry.
| Growth factor | Observed mass, non-reduced form | Observed mass, reduced form | Cleavage site | Comments |
|---|---|---|---|---|
| FGF2 | 18295.1 | ↓ST-APALPED….. | ||
| 18208.4 | ↓T-APALPED…. | |||
| 18107.3 | ↓APALPED…. | Mature Ala-FGF2 | ||
| 17939.3 | ↓ALPED…. | |||
| IGF1 | 9277.6 | 9283.4 | ↓GPETLC…. | Mature IGF1 |
| 9186.8 | ↓GPETLC…. | IGF1 (K102 truncation) methionine sulfone | ||
| 9166.3 | 9171.9 | ↓GPETLC…. | IGF1 (K102 truncation) methionine sulfoxide | |
| 9149.3 | 9155.2 | ↓GPETLC…. | IGF1 (K102 truncation) | |
| 9139.3 | Impurity | |||
| 9123.0 | 9123.2 | Impurity | ||
| 9010.9 | Impurity |