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. 2017 Nov 3;7:14976. doi: 10.1038/s41598-017-14549-9

Figure 5.

Figure 5

EPHA4 is required for TGFβ-mediated Migration in Human Breast Cancer Cell Lines. (A) Stable MDA-MB-231 and SCP2 cell infected with a scrambled or EPHA4 specific shRNA were seeded to confluence in 6-well plates. Migration was measured following treatment with 0 or 5 ug/mL of TGFβ1 for 24 hours using a wound-healing migration assay. (B) Wound-closure is graphed relative to the shRNA condition and represents the mean from 3 independent experiments. Pictures are representative of the wound for the indicated conditions at 0 and 24 hours after TGFβ treatment. (C) Efficiency of EPHA4 knockdown in MDA-MB-231 and SCP2 cell after shRNA infection. EPHA4 expression was measured by qPCR. (D) Efficiency of the EPHA4 knockdown in MDA-MB-231 and SCP2 cells following viral siRNA and shRNA infection. EPHA4 expression was measured by western blot.